Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2016 Springer Science+Business Media New York 2016 RNAseq.estrogen recepto

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Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors,omoters of estrogen-dependent genes is a common mechanism to activate or enhance gene transcription in breast cancer thus promoting tumor progression. In this chapter, we demonstrate a stepwise protocol for ChIP assay using binding of ERα to its genomic targets after stimulation with 17β-estradiol (