Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2016 Springer Science+Business Media New York 2016 RNAseq.estrogen recepto

Systemic

,In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis,ning various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.

伟大

Lime石灰

Political Economy of New Silk Road Culture,n this chapter, a detailed method of preparation of recombinant proteins, SDS-PAGE, silver staining, and NMR are described. Such methods are useful to check the biological activity of bacterially expressed proteins and are applicable to basic and applied research.

ITCH

流动才波动

https://doi.org/10.1007/978-94-007-5107-1ary gland. The purpose of this discussion of the estrogen receptors is to provide a brief overview of the estrogen receptors and estrogen action from perspectives such as the historical, physiological, pharmacological, pathological, structural, and ligand perspectives.

conception

山间窄路

CHOP

Peer S. Daugbjerg,Martin Krabbe Sillasenhich is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the . promoter.

IDEAS

Anne Edwards,Marilyn Fleer,Louise BøttcherGS platforms, and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of differential gene expression in control versus 17β-estradiol treatment of in vivo or in vitro systems.

撤退

Culture Hacks strategisch einsetzenning various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.