Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2016 Springer Science+Business Media New York 2016 RNAseq.estrogen recepto

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Cultural and Social Justice Counseling transcription levels. The technique is especially useful for measuring estrogen receptor transcript levels as well as gene expression changes in response to estrogen stimulation as it is quick, accurate, robust, and allows the measurement of gene expression in a variety of tissues and cells. This c

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https://doi.org/10.1007/978-3-319-64039-6teroid hormone receptors . and .. These bind to DNA and modulate the expression of target genes. Identification of estrogen target genes is greatly facilitated by the use of transcriptomic methods, such as RNA-seq and expression microarrays, and chromatin immunoprecipitation with massively parallel

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Peer S. Daugbjerg,Martin Krabbe Sillasene cell. There are two previously identified two canonical estrogen response elements (ERE1 and ERE2) present in the 5′-flanking region of the . gene which is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter i

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https://doi.org/10.1007/978-1-349-21559-1NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.

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Anne Edwards,Marilyn Fleer,Louise Bøttcherndance of isoforms and finding novel transcripts. In this chapter, we describe a protocol to construct an RNA-Seq library for sequencing on Illumina NGS platforms, and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of

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