Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2016 Springer Science+Business Media New York 2016 RNAseq.estrogen recepto

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Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G-Protein-Coupled Estroges are essential to elucidate the underlying effects of this novel estrogen metabolite and to validate its targets; therefore, this competitive receptor-binding assay protocol was developed in order to assess the membrane receptor binding and affinity of 2-methyoxyestradiol.

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Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromato rat uterus after estrogen (ethinylestradiol) treatment. The steps of the protocol involve sample preparation (digestion), 2D-nanoLC-MS/MS analysis, and shotgun proteomics analysis including bioinformatics tools for data conversion, organization, and interpretation.

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Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction Between the Estroly structured in α-helix, is a key coactivator for ERα activity. Here, we show how circular dichroism can be used to study the interaction of ERα with Ca.-calmodulin. Our approach allows the determination not only of the conformational changes induced upon complex formation but also the dissociation constant (.) of this interaction.

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Book 2016n this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen rece

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Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of , in TM4 Sertoli Cells,hich is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the . promoter.

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