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Titlebook: Environmental Microbiology; Methods and Protocol John M. Walker,John F. T. Spencer,Alicia L. Ragout Book 2004 Humana Press 2004

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Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic Acid Bacteriaication as starter cultures in the food industry (., .). Milk has been commonly used as a culture medium for EPS production. However, the isolation of polysaccharides from coagulated milks is tedious, time-consuming, and includes the risk of degrading the polymer during manipulation (.). When a comp
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Determination of Oligosaccharides in Fermented Soymilk Products by High-Performance Liquid Chromatog consumer acceptability owing to the presence of high levels of undigested sugars (e.g., stachyose, raffinose). These oligosaccharides are degraded by the human intestinal bacteria to carbon dioxide, hydrogen, and methane, which produce flatulence and abdominal pain (.). Therefore, processes includi
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Evaluation of Minimal Nutritional Requirements of Lactic Acid Bacteria Used in Functional Foodsion, it has been showed that LAB exhibit a range of physiological and therapeutic effects in consumers of these foods, including immune stimulation, pathogen exclusion, production of bioactive peptides, and others (.). Foods containing such bacteria are considered “functional foods,” foods that prom
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A Simple Method for Obtaining DNA Suitable for RAPD Analysis From ,e for the analysis of genomic polymorphism among related organisms. This method proved to be particularly useful in genomic fingerprinting and phylogenetic studies of microorganisms that present taxonomic problems or a large diversity within the taxon, such as clinical and soil microorganisms (.–.).
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PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmentaly, these methods presently dominate modern taxonomic studies as a consequence of technological progress, but primarily because the present view on classification is that it should reflect the natural relationships as encoded in the DNA. Some of these methods are not fully sequence-based, although in
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Book 2004,” “Recovery and Determination of Nucleic Acids,” and the review s- tion, containing chapters on the endophytic bacterium, Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, using the hemoglobin gene from a strain of Vitreosci
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