Titlebook: Environmental Microbiology; Methods and Protocol John M. Walker,John F. T. Spencer,Alicia L. Ragout Book 2004 Humana Press 2004

Preamble

Reverse Sample Genome Probing to Monitor Microbial Communitiesnity composition. A variety of methods to monitor environmental microbial diversity exist, e.g., the use of 16S rRNA probes, combined with fluorescence . hybridization (FISH), or fatty acid methylester analysis. However, no currently available method completely overcomes the difficulties presented b

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Assessing Bacterial DNA Content in Aquatic Systemssamples and cultures (.). Apparent DNA content is a valuable tool when used in conjunction with forward light scatter for biomass (.–.) to characterize heterotrophic bacterioplankton, organisms which are too small for observation by light microscopy and difficult to cultivate (.).

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Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspten, it is desirable to be able to identify and measure the amount of a particular analyte DNA in a mixture. When there are multiple analyte DNAs of interest, multiplexing techniques can speed up the analysis.

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Molecular Characterization of Microbial Communities From Marine Environmentscoplankton (.). Marine prokaryotes are considered to be the major primary producers and heterotrophic consumers in these systems (.). Ferguson et al. showed that more than 99.9% of the natural bacterioplankton community in seawater could not be cultured on marine agar 2216 medium (.).

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Yeastsractical value and as the fundamental basis of their evolution. New species of yeast are formed due to selection pressures exerted by the environment, and observation of the similarities and differences among the yeasts found in a particular environment can assist the investigator to see species for

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Aeromonads in Environmental Watersquatic systems; for this reason, the potential public health significance cannot be ignored (.). Their presence has been reported in sewage and sewage effluents, surface waters (fresh, estuarine, and marine), fish ponds, soils, natural mineral springs, stagnant water, chlorinated and unchlorinated d

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Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface val, movement, and growth of bacteria in a variety of environments and under a range of conditions. For field-scale studies, the methods have mainly focused on the use of nucleic-acid-binding fluorescent stains, most of which have detrimental effects on cell properties and functions, and also pose a

maintenance

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