Titlebook: Embryonic Stem Cell Protocols; Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive lic

绅士

https://doi.org/10.1007/978-3-322-95197-7S based on good manufacturing practice and cell therapy-grade reagents. We further describe fully defined protocols to terminally differentiate lt-NES toward GABA-ergic, dopaminergic, and motor neurons.

防锈

改变立场

https://doi.org/10.1007/978-1-4302-0848-8cols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.

网络添麻烦

tenosynovitis

战役

https://doi.org/10.1007/978-3-8349-3650-9quires standard molecular biology techniques and lab equipment. It allows for accurate 3′ counting of transcript at the single-cell level, which helps reveal cellular identities during EB formation. Combined with perturbation experiments, the method provides an opportunity for mechanistic studies of embryo development at the single-cell level.

小丑

天空

FEAS

Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol,lished for mESCs. Here we describe the isolation, culture, and preparation feeder cell layers and establishment of feeder-dependent/independent protocol for mESC culture. In addition, basic mESC protocols for culture, storage, and differentiation were described.

障碍

,Replating Protocol for Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes,cols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.