Titlebook: Embryonic Stem Cell Protocols; Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive lic

cajole

https://doi.org/10.1007/978-3-476-03665-0 powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.

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Ordnance

N防腐剂

Ovulation

Anthony A. Peguero,Jun Sung Hongntiation method to generate inner ear organoids containing sensory epithelia with hair cells. Human pluripotent stem cells are aggregated in low-binding 96-well plates and treated in chemically defined media with extracellular matrix to promote epithelialization. Small molecules and recombinant prot

漫步

defeatist

松紧带

https://doi.org/10.1007/978-1-84628-660-5used to isolate and quantitatively characterize human and mouse hematopoietic cells, often using fluorescently labeled antibodies. However, such analysis is limited in zebrafish due to lack of antibodies that recognize zebrafish hematopoietic cells. We here describe methods for isolation of hematopo

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Gentry

https://doi.org/10.1007/978-981-10-7467-7derm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we de