Titlebook: Embryonic Stem Cell Protocols; Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive lic

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https://doi.org/10.1007/978-3-8349-3650-9mbryonic stem cells (ESCs), which serves as a useful model for understanding early embryo development. Due to the mixture of different cell types, it is necessary to investigate EBs at the single-cell level. Here, we describe a robust and straightforward method for single-cell gene expression profil

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https://doi.org/10.1007/978-3-658-20614-7tiate into cells of all three germ layers (pluripotency). These unique properties make them exceptionally valuable in basic science and clinical researches, including cell replacement therapies, drug discovery, and regenerative medicine. Mouse ESCs represent an important model system for studying ge

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Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids,ells, as well as neurons with synaptic formations to hair cells. This human stem cell–derived inner ear organoid system provides an ideal platform to study human inner ear development and disease in vitro.

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Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs), Metabolic Assay. This may be a useful protocol for understanding how the glycolytic function of mESCs changes in certain circumstances and how is it coupled to diverse pluripotency/differentiation phenotypes.

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