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派博传思国际中心 »期刊书目

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Titlebook: Embryonic Stem Cell Protocols; Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive lic

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发表于 2025-3-21 18:42:22 | 显示全部楼层 |阅读模式
书目名称Embryonic Stem Cell Protocols
编辑Kursad Turksen
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Embryonic Stem Cell Protocols; Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive lic
描述This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Embryonic Stem Cell Protocols, Fourth Edition. serves as an ideal resource for researching mining the depths of the ESC field..
出版日期Book 2022Latest edition
关键词Stem cells; Induced pluripotent stem cells; Cell lineage; Organoids; ESCs
版次4
doihttps://doi.org/10.1007/978-1-0716-2437-1
isbn_softcover978-1-0716-2439-5
isbn_ebook978-1-0716-2437-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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发表于 2025-3-22 02:59:28 | 显示全部楼层
https://doi.org/10.1007/978-1-0716-2437-1Stem cells; Induced pluripotent stem cells; Cell lineage; Organoids; ESCs
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发表于 2025-3-22 05:43:45 | 显示全部楼层
https://doi.org/10.1007/978-3-476-03665-0 powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.
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chemoprevention
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978-1-0716-2439-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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发表于 2025-3-22 20:36:46 | 显示全部楼层
Embryonic Stem Cell Protocols978-1-0716-2437-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
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发表于 2025-3-23 00:48:08 | 显示全部楼层
https://doi.org/10.1007/978-3-322-83289-4a highly multiplexed manner. A large number of genes are perturbed in a cell population through genomic integration of one single-guide RNA (sgRNA) per cell. A subset of cells with the phenotype of interest can then be enriched through fluorescence-activated cell sorting (FACS). SgRNAs with altered
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https://doi.org/10.1007/978-981-10-8087-6lture is an important milestone for human pluripotent stem cell research which eliminates the feeder cells. Various matrices and medium formulations have been generated for feeder-independent culture. Here we described an mTeSR™1 based feeder-independent human ES cell culture on Matrigel. matrix. Cu
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发表于 2025-3-23 05:50:23 | 显示全部楼层
ewal and pluripotency. These cells have broad differentiation capability to convert into diverse cell types that make up the primary germ layers during embryonic development. iPS cells can spontaneously differentiate and form cell aggregates termed embryoid bodies (EBs) in the absence of differentia
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