Titlebook: Effector-Triggered Immunity; Methods and Protocol Thomas A. Kufer,Maria Kaparakis-Liaskos Book 2022 The Editor(s) (if applicable) and The A

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要素

Functional Assessment of Disease-Associated Pyrin Variants, pyroptosis. Ancient plague pandemics in the Mediterranean basin have selected in the human population pyrin variants that can trigger an autoinflammatory disease termed familial Mediterranean fever (FMF). In addition, distinct mutations in ., the gene encoding pyrin, cause a different rare autoinfl

Systemic

anaerobic

Detection of Gasdermin Activation and Lytic Cell Death During Pyroptosis and Apoptosis, the assembly of inflammasomes, multimeric protein complexes that drive caspase-1 activation. Active caspase-1 cleaves the proinflammatory cytokines IL-1β and IL-18 and the pore-forming protein gasdermin-D (GSDMD) thereby liberating its N-terminal domain. The GSDMD N-termini form multimeric pores at

certain

Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging,ates the NLRP3 inflammasome to induce host pore-forming proteins and cellular suicide, termed pyroptosis. Host cell death rates are routinely determined at pre-defined time points and on whole cell populations. To capture the dynamic interactions between bacterial pore-forming toxins and host cell d

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ACTIN

indubitable

Quantifying Regulated Mitochondrial Fission in Macrophages,r being tasked with energy generation through the production of adenosine triphosphate, but they also have roles in several other cellular processes, for example, immune and inflammatory responses. Mitochondria have important functions in macrophages, key innate immune cells that detect pathogens an

Commodious

,Live Cell Imaging of T Cell Pyroptosis, is best characterized in myeloid cells, but there is growing evidence that this cell death program also functions in human T cells. Several studies have suggested a role for inflammasome components in T-cell biology but often do not unambiguously clarify whether this means that T cells progress to

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