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Titlebook: Effector-Triggered Immunity; Methods and Protocol Thomas A. Kufer,Maria Kaparakis-Liaskos Book 2022 The Editor(s) (if applicable) and The A

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书目名称Effector-Triggered Immunity
副标题Methods and Protocol
编辑Thomas A. Kufer,Maria Kaparakis-Liaskos
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detailed essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Effector-Triggered Immunity; Methods and Protocol Thomas A. Kufer,Maria Kaparakis-Liaskos Book 2022 The Editor(s) (if applicable) and The A
描述This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key host responses; and techniques used for the analysis of inflammasome activation, cell death pathways, and mitochondria damage in response to pathogens. All of these topics cover a broad spectrum of immunological, biochemical, cell biological, and structural biology approaches to examine ETI. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and practical, .Effector-Triggered Immunity: Methods and Protocols. is a valuable resource for both expert and novice researchers who are interested in learning more about the important and developing field of ETI..
出版日期Book 2022
关键词Pathogen-associated molecular patterns; ETI; Pathogen-derived effector proteins; Immune responses; Plant
版次1
doihttps://doi.org/10.1007/978-1-0716-2449-4
isbn_softcover978-1-0716-2451-7
isbn_ebook978-1-0716-2449-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Lambda Red Recombineering in ,d by an incomplete understanding of the molecular mechanisms by which . spp. causes disease and difficulties in manipulating . spp. genomes. While homologous recombination protocols for the construction of precise gene deletions exist, construction of mutants in . has not become commonplace. We desc
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Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of erial effector molecules, and can trigger a range of responses in the host. The study of BMV production, composition, and functions can give insights into their roles in mediating bacterial survival, pathogenesis, and disease. Furthermore, BMVs can be harnessed to develop cutting-edge nano-therapeut
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Host-Induced Gene Silencing Using BPMV on Soybean to Study Genes in the Soybean Rust Fungus ,or silence the respective gene. Since genetic transformation is not yet possible for rust fungi, silencing the gene is the only option. Posttranscriptional gene silencing uses RNAi. RNAi in plant pathogens can be accomplished by introducing dsRNA either by direct application of in vitro synthesized
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