Titlebook: Effector-Triggered Immunity; Methods and Protocol Thomas A. Kufer,Maria Kaparakis-Liaskos Book 2022 The Editor(s) (if applicable) and The A

PACT

https://doi.org/10.1007/978-3-642-71980-6dsRNA or through positive-strand or double-strand RNA plant viruses. For studying effectors in ., we have implemented a host-induced silencing procedure based on virus-induced gene silencing using the bean pod mottle virus system. Here, procedures and interpretations of results are described and limitations of the system are discussed.

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Host-Induced Gene Silencing Using BPMV on Soybean to Study Genes in the Soybean Rust Fungus ,dsRNA or through positive-strand or double-strand RNA plant viruses. For studying effectors in ., we have implemented a host-induced silencing procedure based on virus-induced gene silencing using the bean pod mottle virus system. Here, procedures and interpretations of results are described and limitations of the system are discussed.

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Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1hermophoresis (MST) for efficient one-step purification of NOD1-GFP and NOD2-GFP and easy measurement of the binding affinities of NOD1 or NOD2 with sphingosine-1-phosphate (S1P) using small amount of proteins (nM range). This method will allow the identification of novel agonists/antagonists for NOD1/2.

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A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Assocird immobilization strategy in surface plasmon resonance experiments. With this highly sensitive and label-free technique, the kinetics and affinities of NLR and co-factor interactions can be measured directly at the protein level.

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