Titlebook: Drug‘DNA Interaction Protocols; Keith R. Fox Book 19971st edition Humana Press 1997

muster

主讲人

Strong, Em, and Other Phrase Elements local DNA conformation and/or mask potential binding sites. This chapter describes the preparation of nucleosome particles containing radiolabeled DNA fragments that can be used as substrates for footprinting experiments.

较早

Quantitative DNA Footprinting,, electrophoresis in a polyacrylamide gel. If the ligand, when bound, inhibits cleavage by the probe, the oligonucleotides that terminate at the ligand binding site will be underrepresented among the products analyzed using the sequencing gel. This appears as omissions or “footprints” in the spots on the sequencing autoradiogram

OWL

,Uranyl Photoprobing of DNA Structures and Drug–DNA Complexes,tion sites of various proteins, such as the λ-repressor/O. complex (.), RNA polymerase-.P1 promoter of . (.), RNA polymerase/cyclic AMP receptor protein (CRP)-.P2 promoter of . (.), CRP/CytR repressor-.P2 promoter of . (.), and transcription factor IIIA/. 5s internal control region (.).

Morbid

alabaster

Mri485

The Ins and Outs of the Third Dimensionssful studies have been reported for both RNA and DNA systems, including condensed triple- and four-stranded systems. The present discussion, restricted to examples involving duplex DNA, illustrates the general principles involved in these powerful optical techniques.

anus928

,Optical Absorbance and Fluorescence Techniques for Measuring DNA–Drug Interactions,ssful studies have been reported for both RNA and DNA systems, including condensed triple- and four-stranded systems. The present discussion, restricted to examples involving duplex DNA, illustrates the general principles involved in these powerful optical techniques.

DOSE

吞没

https://doi.org/10.1007/978-1-4302-1921-7, electrophoresis in a polyacrylamide gel. If the ligand, when bound, inhibits cleavage by the probe, the oligonucleotides that terminate at the ligand binding site will be underrepresented among the products analyzed using the sequencing gel. This appears as omissions or “footprints” in the spots on the sequencing autoradiogram