Titlebook: Drug‘DNA Interaction Protocols; Keith R. Fox Book 19971st edition Humana Press 1997

药物

Strong, Em, and Other Phrase Elementsses are oxidized by the photochemically excited state of the uranyl1 ion, a very strong oxidant (.). Thus the uranyl ion is an efficient DNA photocleavage reagent (.,.) that has been used to study the sequence specific interaction with the phosphates of the DNA backbone of the respective DNA recogin

Polydipsia

Strong, Em, and Other Phrase Elementscleic acids (.). Secondary structures of native DNA and RNA as well as ligandinduced changes in conformation can be probed by the use of a variety of chemical reagents, either in vitro with purified nucleic acids in a reconstituted acellular environment, or directly within the framework of a cell Ov

条街道往前推

Strong, Em, and Other Phrase Elementsively concerned their binding to naked DNA. In contrast, cellular DNA is packed into chromatm, generating higher order structures, which may alter the local DNA conformation and/or mask potential binding sites. This chapter describes the preparation of nucleosome particles containing radiolabeled DN

Itinerant

enchant

fringe

grotto

Strong, Em, and Other Phrase Elementso interact with DNA, either by intercalation (e.g., doxorubicin, mitoxantrone), groove binding (e.g., distamycin), formation of adducts or crosslinks (e.g., melphalan, cisplatin, mitomycin C), or by incorporation of modified bases (e.g., 5-fluorouracil, 6-thioguanine). There has therefore been a gre

Radiation

Strong, Em, and Other Phrase Elementsh are known to bind covalently to, and in many cases crosslink, the bases of DNA. These include the nitrogen mustard, chloroethyl-nitrosourea, triazene, and dimethanesulfonate classes of agents. Most simple alkylating agents of this type show a base specificity for guanine, binding primarily to the

accordance

New Window on the World with OpenGL ESe, but they are involved in the pathogenesls of the disease through the formation of immune complexes that indirectly cause tissue damage. Alternatively, the antibodies may crossreact with cell surface antigens and cause tissue damage directly. Thus, understanding the specificity of autounmune antib

Ventricle

Further OpenGL Features and Techniquesfic antrbody. Most DNA is not immunogeinc, although DNA-binding antibodies are produced spontaneously in the autoimmune drsease systemrc lupus erythematosus (.). In general, these antibodies exhtbtt little sequence or structural specificity and, therefore, are not useful for probing chromosomes exce