Titlebook: DNA and RNA Isolation Techniques for Non-Experts; Akash Gautam Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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978-3-030-94232-8The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl

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Basic Idea About Nucleic Acids for NonexpertsNA is mainly involved in the processing (transcription) and manifestation (translation) of this genetic information. They are unbranched macromolecules made up of monomeric units called nucleotides which are linked together by phosphodiester bonds. Nucleotides consist of a pentose sugar, a nitrogeno

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General Steps During Isolation of DNA and RNAerial is present either as DNA or RNA inside cells. The biological study of these DNA or RNA molecules for various purposes requires their isolation from their native location to the in vitro conditions. Over the years, scientists have developed different extraction techniques for DNA and RNA from a

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Phenol-Chloroform DNA Isolation Methodmolecular weight DNA from any biological sample using a mixture of phenol-chloroform-isoamyl alcohol. The basic principle involves the separation of DNA, RNA, and proteins in a sample, based on the differential solubilities of these molecules in different immiscible liquids. The isolated DNA can be

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RNA Isolation by the Guanidinium-Acid-Phenol Methodwn as the Trizol method of RNA isolation. During this method, the sample is treated with guanidinium isothiocyanate and acidic phenol, and chloroform. Here guanidinium isothiocyanate acts as a powerful protein denaturant and deactivates the RNases, whereas the acidic phenol-chloroform helps in the p

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Spin Column-Based Isolation of Nucleic Acid tissues, plant extracts, viruses, and bacteria. It is advantageous over other extraction techniques such as less time consumption, economical, energy-efficient, automated operation, prevention from cross-contamination, and high yield. It is based on the principle of binding nucleic acid to immobili

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Isolation of Plasmid DNA by Alkaline Lysisthe chromosomal DNA. They provide antibiotic resistance and hence better adaptation qualities to these microorganisms to survive in different environmental conditions. Molecular biologists harness these properties of plasmid DNA and use them for several applications such as sequencing, screening clo

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DNA Isolation by Hydrophilic Ionic Liquid Treatments only a few minutes to complete. Ionic liquids can dissolve biomass and release DNA molecules from tissues and cells. DNA released in the supernatant may be used as such for downstream applications. The extraction procedure includes mixing ionic liquids with starting sample at a high temperature (6

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