Titlebook: DNA and RNA Isolation Techniques for Non-Experts; Akash Gautam Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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Philip Quinn,Wenxin Feng,Shumin ZhaiNA is mainly involved in the processing (transcription) and manifestation (translation) of this genetic information. They are unbranched macromolecules made up of monomeric units called nucleotides which are linked together by phosphodiester bonds. Nucleotides consist of a pentose sugar, a nitrogeno

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Xucong Zhang,Seonwook Park,Anna Maria Feiterial is present either as DNA or RNA inside cells. The biological study of these DNA or RNA molecules for various purposes requires their isolation from their native location to the in vitro conditions. Over the years, scientists have developed different extraction techniques for DNA and RNA from a

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Poulami Majumder,Partha Pratim Ray tissues, plant extracts, viruses, and bacteria. It is advantageous over other extraction techniques such as less time consumption, economical, energy-efficient, automated operation, prevention from cross-contamination, and high yield. It is based on the principle of binding nucleic acid to immobili

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Applications: Physical Toy Examplesdoes not involve any harmful organic solvents. The basic protocol involves the extraction of DNA by adding samples to hot Chelex suspensions at pH 10–11. The alkalinity of resin suspension and exposure to heat result in disruption of the cell membrane. Heating also causes the double helix of DNA to