Titlebook: Coronaviruses; Methods and Protocol Helena J. Maier,Erica Bickerton Book 2020Latest edition Springer Science+Business Media, LLC, part of S

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Web-based Virtual Research Environmentsst in 2012. Serological assays are used to diagnose MERS-CoV infection and to screen for serum antibodies in seroepidemiological studies. The conventional enzyme-linked immunosorbent assay (ELISA) is the preferred tool for detecting serum antibodies specific for pathogens; however, the utility of co

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Web-based Virtual Research Environmentsgnificant depth of coverage. The protocol describes sequencing of infectious bronchitis virus propagated in embryonated eggs and harvested in the allantoic fluid. The protocol is composed of three main steps—enrichment of the allantoic fluid using ultracentrifugation, extraction of total RNA from al

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Supporting Web Search with Visualizationrsing piglets. PDCoV-related diarrhea first occurred in the USA in 2014 and was subsequently reported in South Korea, China, Thailand, Vietnam, and Lao People’s Democratic Republic, leading to massive economic losses and posing a threat to the swine industry worldwide. Currently, no treatments or va

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Wetlands and Sustainable Drainage,mbryo and its membranes provide a diversity of cell types that allow for the successful replication of a wide variety of different viruses. Within the family . the embryonated egg has been used as a host system primarily for two avian coronaviruses within the genus ., infectious bronchitis virus (IB

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Introduction to Wetland Systems,including coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host–pathogen interactions in animal reservoir host species. In this chapter, we provide a

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Wetlands and Sustainable Drainage,technologies improving old techniques or generating new ones, generally relying on partial detection methods or structural analysis. Therefore, characterization of virus infectivity in a sample is often essential, and classical virological methods are extremely powerful in providing accurate results