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Titlebook: Coronaviruses; Methods and Protocol Helena J. Maier,Erica Bickerton Book 2020Latest edition Springer Science+Business Media, LLC, part of S

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发表于 2025-3-21 16:09:53 | 显示全部楼层 |阅读模式
书目名称Coronaviruses
副标题Methods and Protocol
编辑Helena J. Maier,Erica Bickerton
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Coronaviruses; Methods and Protocol Helena J. Maier,Erica Bickerton Book 2020Latest edition Springer Science+Business Media, LLC, part of S
描述This detailed new edition provides a comprehensive collection of protocols applicable to all members of the .Coronavirinae. sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and cutting-edge, .Coronaviruses: Methods and Protocols, Second Edition. serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals..
出版日期Book 2020Latest edition
关键词Coronavirinae sub-family; SARS; Virus propagation; Antiviral therapeutics; Virus-host interactions; Diagn
版次2
doihttps://doi.org/10.1007/978-1-0716-0900-2
isbn_softcover978-1-0716-0902-6
isbn_ebook978-1-0716-0900-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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发表于 2025-3-22 00:18:46 | 显示全部楼层
Quantification of Coronaviruses by Titration In Vitro and Ex Vivo even in an old-fashioned way. In this chapter, we describe in detail the techniques routinely used to estimate the number of viable infectious coronavirus particles in a given sample. All these techniques are serial dilution assays, also known as titrations or end-point dilution assays (EPDA).
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Detection and Discovery of Coronaviruses in Wild Bird Populationst sequencing technologies to obtain their entire genome, which overcomes the inherent difficulties of wild bird coronavirus sequencing, that is, their genetic diversity and the lack of virus isolation methods.
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Wetlands and Sustainable Drainage,vealed by observation of ciliostasis as marker of infection in tracheas extracted from birds ex vivo. as well as in vitro analysis providing a reliable infection model and a useful tool for titration.
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发表于 2025-3-23 02:54:09 | 显示全部楼层
Mohammad Nauman,Shahbaz Khan,Sanaullah Khanhat they are the natural reservoirs of the virus. In this chapter we describe the process of screening rodent tissue for novel coronaviruses with PCR, a method that is easily adaptable for screening a range of animals.
发表于 2025-3-23 07:20:44 | 显示全部楼层
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