Titlebook: Coronaviruses; Methods and Protocol Helena J. Maier,Erica Bickerton Book 2020Latest edition Springer Science+Business Media, LLC, part of S

ALOFT

Ceremony

微粒

Web-based Virtual Research Environmentst competes with serum antibodies for binding to an antigen; therefore, it is used widely for serological surveillance of many pathogens. In this chapter, I describe detection of serum antibodies using cELISA based on MAbs specific for MERS-CoV.

antecedence

Chromatic

附录

https://doi.org/10.1007/978-1-935704-00-3no specific equipment and can be performed in a standard laboratory setting. The protocol described can be easily adapted to virtually all known or emerging coronaviruses, such as Middle East respiratory syndrome coronavirus (MERS-CoV).

Corral

Sverre Petterssen,James Rodger Flemingof viral proteins, it can be used for any current or emerging virus, regardless of biocontainment requirements and ability to culture the virus. In this chapter, we will outline the protocols that can be used to take advantage of . as a tool to advance understanding of how viruses interact with eukaryotic cells.

POWER

Sinus-Rhythm

Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndromt competes with serum antibodies for binding to an antigen; therefore, it is used widely for serological surveillance of many pathogens. In this chapter, I describe detection of serum antibodies using cELISA based on MAbs specific for MERS-CoV.

格言

Isolation and Propagation of Coronaviruses in Embryonated Eggsd allantoic fluids. TCoV replicates only in epithelium lining the embryo intestines and bursa of Fabricius; thus, amniotic inoculation is required for isolation and propagation of this virus. Embryonated eggs also provide a potential host system for detection, propagation, and characterization of other, novel coronaviruses.