Titlebook: Coronaviruses; Methods and Protocol Helena J. Maier,Erica Bickerton Book 2020Latest edition Springer Science+Business Media, LLC, part of S

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Well-Differentiated Primary Mammalian Airway Epithelial Cell Culturesincluding coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host–pathogen interactions in animal reservoir host species. In this chapter, we provide a

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In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipelinestems, however, present several limitations and are sometimes difficult to establish in a timely manner for (re-)emerging viruses. In this chapter, we present a new universal reverse genetics platform for the assembly and engineering of infectious full-length cDNAs using yeast-based transformation-a

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Proximity Labeling for the Identification of Coronavirus–Host Protein Interactionsf enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronaviru

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Using Yeast to Identify Coronavirus–Host Protein Interactionsvarious advantages, but in the context of coronavirus research and the system outlined here, they are simple and easy to work with and can be used at biosafety level 2. The system involves inducible expression of individual viral proteins and identification of detrimental phenotypes in the yeast. Ye

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https://doi.org/10.1007/978-1-0716-0900-2Coronavirinae sub-family; SARS; Virus propagation; Antiviral therapeutics; Virus-host interactions; Diagn

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Coronaviruses978-1-0716-0900-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-1-84882-628-1RNA genome, and a unique replication strategy. CoVs cause a variety of diseases in mammals and birds ranging from enteritis in cows and pigs, and upper respiratory tract and kidney disease in chickens to lethal human respiratory infections. Most recently, the novel coronavirus, SARS-CoV-2, which was