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Titlebook: Contemporary Approaches to the Study of Pain; From Molecules to Ne Rebecca P. Seal Book 2022 Springer Science+Business Media, LLC, part of

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发表于 2025-3-21 17:14:34 | 显示全部楼层 |阅读模式
书目名称Contemporary Approaches to the Study of Pain
副标题From Molecules to Ne
编辑Rebecca P. Seal
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Neuromethods
图书封面Titlebook: Contemporary Approaches to the Study of Pain; From Molecules to Ne Rebecca P. Seal Book 2022 Springer Science+Business Media, LLC, part of
描述.This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates;  the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning.  In the .Neuromethods. series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory..Cutting-edge and comprehensive, .Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks .is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain..
出版日期Book 2022
关键词Opioids; Chemogenetics; electrophysiology; calcium imaging; hESC
版次1
doihttps://doi.org/10.1007/978-1-0716-2039-7
isbn_softcover978-1-0716-2041-0
isbn_ebook978-1-0716-2039-7Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
The information of publication is updating

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Visualizing Synaptic Connectivity Using Confocal and Electron Microscopy : Neuroanatomical Approachpter are to provide detailed descriptions of some of these protocols, to highlight both advantages and potential difficulties that may be encountered when using these approaches, and to reference several studies where these have been applied to study spinal circuits in normal and chronic pain states.
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Optical Imaging of the Spinal Cord for the Study of Pain : From Molecules to Neural Networks,alized in these preparations as can the inputs from primary sensory neurons. We also include a detailed protocol used by our laboratory to monitor cells in laminae I and II outer in mice as well as a discussion of the limitations of cell imaging in the spinal cord.
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Nachweis und Bestimmung von Fermenten,nd how they may influence sensory transduction. In this chapter, we describe a powerful electrophysiological technique that allows for ex vivo recordings from intact primary muscle afferents from two target muscle groups, similar to that previously described for cutaneous afferents.
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https://doi.org/10.1007/978-3-663-04972-2e the lumbar spinal cord by laminectomy and then stereotaxically inject the dissociated cells into the dorsal horn. Our studies have demonstrated that 2 weeks posttransplantation, MGE cells are fully differentiated into mature GABAergic interneurons and have functionally integrated into the host spinal circuitry.
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Wilhelm Sturtzel,Hermann Schmidt-Stiebitzenerate a Type17 immune response and that this response was protective against the spread of injury (Cohen et al. Cell 178(4):919–32 e14, 2019). Here, we describe the mouse models, stimulation protocols and equipment used in these studies.
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Single-Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons Using a Novel Ex Vivond how they may influence sensory transduction. In this chapter, we describe a powerful electrophysiological technique that allows for ex vivo recordings from intact primary muscle afferents from two target muscle groups, similar to that previously described for cutaneous afferents.
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