Titlebook: Contemporary Approaches to the Study of Pain; From Molecules to Ne Rebecca P. Seal Book 2022 Springer Science+Business Media, LLC, part of

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https://doi.org/10.1007/978-3-663-04976-0oped techniques utilizing optogenetic mouse models. These techniques will enable researchers to define how subtypes of afferent neurons and nonneuronal cell populations contribute to visceral pain in both normal and pathological conditions. This chapter details how optogenetics can be integrated int

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Single-Cell RNA Sequencing of Somatosensory Neurons,ns limited. However, single-cell transcriptomics has provided a completely new insight into the cellular complexity of sensory tissue. Thus, recent publications describe the transcriptome of spinal cord and dorsal root ganglia including the expression profile of cell types. Essential for successful

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Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis,ed as a powerful method to study heterogeneous tissues by allowing for the capture and analysis of individual cells. A key step in the analysis of solid tissues is the dissociation of the tissue into single cells. While many tissues can be readily dissociated by enzymatic or mechanical methods, high

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