Titlebook: Contemporary Approaches to the Study of Pain; From Molecules to Ne Rebecca P. Seal Book 2022 Springer Science+Business Media, LLC, part of

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In Vivo Calcium Imaging of Peripheral Ganglia,ffer significantly to the environments in intact animals. Also, traditional methods like patch clamp electrophysiology are very low throughput. In vivo calcium imaging of peripheral ganglia strives to overcome these limitations. In this chapter, we will discuss the development and procedures associated with this powerful new technique.

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https://doi.org/10.1007/978-3-663-04974-6Neural circuit tracing methods that take advantage of viral vectors and transgenic mice provide opportunities to gain insight into functional connectivity. Here we describe several protocols for identifying synaptic connections made by projection neurons, specific cell types, and neural ensembles.

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Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice,Neural circuit tracing methods that take advantage of viral vectors and transgenic mice provide opportunities to gain insight into functional connectivity. Here we describe several protocols for identifying synaptic connections made by projection neurons, specific cell types, and neural ensembles.

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0893-2336 etworks .is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain..978-1-0716-2041-0978-1-0716-2039-7Series ISSN 0893-2336 Series E-ISSN 1940-6045

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Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve,

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Single-Cell RNA Sequencing of Somatosensory Neurons,ally, we will highlight potential pitfalls and strategies when working with this sensory tissue, in particular spinal cord with its highly sensitive neurons. In summary, we present a comprehensive description on how to perform single-cell RNA-sequencing including suggestions on how to choose and des

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