Titlebook: Clostridium difficile; Methods and Protocol Adam P. Roberts,Peter Mullany Book 2016Latest edition Springer Science+Business Media New York

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Dan Yin,Wengsheng Tang,Peng Chen,Bo Yang bacterial donor and a recipient, both of which are susceptible to infection by the phage of interest. Phage is propagated in the donor, concentrated, and exposed transiently to recipient at different multiplicity of infection ratios. Transductants are selected for the desired phenotype by culture o

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https://doi.org/10.1007/978-3-030-78621-2ribed in this chapter have been designed to investigate the genetic organization of .(B)-containing elements and to evaluate the capability of these elements to transfer in . recipient strains using filter mating assay.

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Weimin He,Lei Zhao,Luofei Cheng including antibiotic resistance. Mobile genetic elements such as conjugative and mobilizable transposons make up a considerable part of the . genome. While sequence analysis has identified a large number of these elements, experimental analysis is required to demonstrate mobility and function. This

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A Review: How to Detect Malicious Domainscytotoxins TcdA and TcdB, was previously hypothesized to be a mobile genetic element; however, mobility was not demonstrated. Here we describe the methods used to facilitate and detect the transfer of the PaLoc from a toxigenic strain into non-toxigenic strains of . Although the precise mechanism of

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Characterization of Functional Prophages in ,,stance genes. There is also some interest in using therapeutic phages to fight . infections..The objective of this chapter is to share with the broader . research community the expertise we developed in the study of . temperate phages. In this chapter, we describe a general “pipeline” comprising a s

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Martin Slota,Martin Baláž,João Leiteore, whereas CLIP is labeled by ..-benzylcytosine conjugates. SNAP and CLIP labeling is orthogonal allowing for dual labeling in the same cells. SNAP and CLIP cassettes optimized for . can be used for quantitative studies of gene expression at the single cell level. Both the SNAP and CLIP reporters