Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo Book 1998 Humana Press 1998

GRIEF

Projection

Long Range PCRThe . polymerase lacks proofreading properties (.) and thus is unable to correct such misincorporations. The higher extension K. value for a misincorporated nucleotide is thought to cause detachment of the . polymerase from template DNA.

外来

陈列

DEI

Nefarious

–DOX

Sequencing of PCR ProductsIt is often necessary to obtain DNA sequence information following successful amplification. Although polymerase chain reaction (PCR) products may be cloned into a vector and sequenced at a later stage, it is often preferable to sequence PCR products directly, without subcloning, and it is this approach that is addressed in this chapter.

不怕任性

antedate

Amplificationtissue specimens. . hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic . (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (.).

概观

PCR Analysis of CD44 Variants in Tumorshain disulfide bonds. It is essentially composed of a 37-kDa protein core highly glycosylated by .- and .-linked sugars to form an 85–90-kDa product and is sometimes additionally linked to chondroitin sulfate side chains to produce a 180–220 kDa form on sodium dodecyl sulfate (SDS) gels (.,.).