Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo Book 1998 Humana Press 1998

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Clinical Applications of PCR978-1-59259-600-3Series ISSN 1543-1894 Series E-ISSN 1940-6037

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https://doi.org/10.1007/978-981-10-6271-1tissue specimens. . hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic . (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (.).

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Julien Bringer,Hervé Chabanne,Linda Guigaegantly simple but the resulting method is extremely powerful. The adoption of the thermostable . polymerase in 1988 greatly simplifies the process and enables the automatron of PCR (.). Since then a large number of applications have been developed that are based on the basic PCR theme. The versatil

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Community Knowledge About Security:ty has also given PCR its main weakness, namely, its tendency to produce false-positive results due to exogenous contamination (.,.). Contamination avoidance is therefore the single most important consideration when setting up a PCR laboratory, especially one concerned with generating diagnostic inf

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Community Knowledge About Security:amplify nucleic acid sequences from archival materials, which in many institutions consist of paraffin-embedded tissue samples (.,.). This ability has allowed the carrying out of large scale retrospective studies of archival materials and has facilitated the use of materials from multiple institutio

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Lecture Notes in Computer Scienceetecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (.). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insu

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https://doi.org/10.1007/978-3-031-61231-2ding even one point mutation. A disadvantage of PCR-SSCP, despite its sensitivity, is the necessity to use radioisotopes. To avoid radioisotopes, silver staining was introduced for band detection (.). In the fluorescent amplification refractory mutation system (ARMS)-SSCP using two different fluores