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Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo Book 1998 Humana Press 1998

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书目名称Clinical Applications of PCR
编辑Y. M. Dennis Lo
视频video
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Clinical Applications of PCR;  Y. M. Dennis Lo Book 1998 Humana Press 1998
描述The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-ba
出版日期Book 1998
版次1
doihttps://doi.org/10.1385/0896034992
isbn_softcover978-0-89603-359-7
isbn_ebook978-1-59259-600-3Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 1998
The information of publication is updating

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https://doi.org/10.1007/978-3-031-61231-2 (Perkin Elmer, Applied Biosystems Division [PE-ABD], Foster City, CA), which can detect four different fluorescent colors in the same lane without controlling gel-temperature. We designed and attached a gel temperature-controlling system to ABI373 A and developed a sensitive method of multiple fluo
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Risks in the Conversion of Energy,ll or organelle. In many cases the level of mutant in an affected tissue correlates well with disease severity. Ideally, a screening test to detect a pathogenic point mutation should not only identify the presence or absence of a pathogenic mutation, but also quantitate the level of the mutation com
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1543-1894 new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-ba978-0-89603-359-7978-1-59259-600-3Series ISSN 1543-1894 Series E-ISSN 1940-6037
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Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal-Labeling (Perkin Elmer, Applied Biosystems Division [PE-ABD], Foster City, CA), which can detect four different fluorescent colors in the same lane without controlling gel-temperature. We designed and attached a gel temperature-controlling system to ABI373 A and developed a sensitive method of multiple fluo
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Diagnosis of Mitochondrial Disorders Using the PCRll or organelle. In many cases the level of mutant in an affected tissue correlates well with disease severity. Ideally, a screening test to detect a pathogenic point mutation should not only identify the presence or absence of a pathogenic mutation, but also quantitate the level of the mutation com
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Risks of Harm from Psychopathic Individualssed into the blood stream as a result of surgery. Detection of circulating tumor cells at diagnosis in early stage patients would imply that the disease is more advanced than indicated by conventional staging. These patients might then be offered adjuvant chemotherapy or radiotherapy post surgery.
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