Titlebook: Circular RNAs; Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu

流浪

https://doi.org/10.1007/978-1-349-08714-3ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdow

Abnormal

Recessive

Joaquín Bernáldez,Rocío Juliana Herrerag sequences to their cognate linear RNAs from the same gene loci, leading to difficulties in distinguishing them from each other. A recent study has shown that some circRNAs can be specifically depleted by using base editing systems to target their predominantly back-splice sites for circularization

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使声音降低

James D. Bradbury,Courtney Cox Smithrpart and can be designed for efficient expression in different cell and tissue types. In this chapter, we developed different backsplicing circRNA cassettes that can enable efficient gene expression in various cell and tissue types. Furthermore, we packaged cassettes encoding circRNAs into adeno-as

Mangle

Brocas-Area

https://doi.org/10.1007/978-1-0716-3678-7splice-aware pseudo-alignment scheme; paired-end RNA-sequencing; ddPCR; CLIP-seq; Bioinformatics

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Christoph Dieterich,Marie-Laure BaudetIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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