Titlebook: Circular RNAs; Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu

撤退

https://doi.org/10.1007/978-3-030-43527-1 in disease pathology. This chapter describes a workflow for investigating the expression profile of EV-circRNAs, which includes EVs separation, library preparation, and bioinformatics analysis. This workflow can aid the investigation of EV-circRNAs and their potential role in disease pathology.

Jejune

Cleveland S. Patterson,Nancy D. Urselng based on the RNA-guided, RNA-targeting CRISPR-Cas13 (RfxCas13d) system. This method can be applied both in vivo and in cell to explore highly expressed circRNAs that may influence cell growth, either under natural conditions or in response to environmental stimulation, without disturbing cognate linear mRNAs.

遗留之物

glomeruli

Jogging

Exploring Circular RNA Profile and Expression in Extracellular Vesicles in disease pathology. This chapter describes a workflow for investigating the expression profile of EV-circRNAs, which includes EVs separation, library preparation, and bioinformatics analysis. This workflow can aid the investigation of EV-circRNAs and their potential role in disease pathology.

UTTER

潜移默化

Base-Editor-Mediated circRNA Knockout by Targeting Predominantly Back-Splice Sites, suggesting an efficient approach for circRNA knockout (KO). Here, we describe the detailed protocol for applying base editors to disrupt back-splice sites of predominantly circularized exons for circRNA KO at the genomic DNA level.

Charitable

上釉彩

Book 2024Latest edition lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls... ..Authoritative and cutting-edge, .Circular RNAs, Second Edition .aims to ensure successful results in the further study of this vital field..

indignant