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Titlebook: Circular RNAs; Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu

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发表于 2025-3-21 19:51:51 | 显示全部楼层 |阅读模式
书目名称Circular RNAs
编辑Christoph Dieterich,Marie-Laure Baudet
视频videohttp://file.papertrans.cn/227/226630/226630.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Circular RNAs;  Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu
描述.This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, .in silico. characterization, circRNA detection, sequence validation, quantification , techniques related to  gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA translation potential, and vaccines based on circular RNAs. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls... ..Authoritative and cutting-edge, .Circular RNAs, Second Edition .aims to ensure successful results in the further study of this vital field..
出版日期Book 2024Latest edition
关键词splice-aware pseudo-alignment scheme; paired-end RNA-sequencing; ddPCR; CLIP-seq; Bioinformatics
版次2
doihttps://doi.org/10.1007/978-1-0716-3678-7
isbn_softcover978-1-0716-3680-0
isbn_ebook978-1-0716-3678-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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书目名称Circular RNAs读者反馈学科排名




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Exploring Circular RNA Profile and Expression in Extracellular Vesiclesotential to modulate biological processes and are being investigated for their diagnostic and therapeutic applications. Circular RNAs (circRNAs), generated through back-splicing of exons, are enriched in EVs. Given their unique characteristics and diverse functions, EV-circRNAs are important players
发表于 2025-3-22 05:13:05 | 显示全部楼层
In Situ Hybridization of circRNAs in Cells and Tissues through BaseScope™ Strategyecules. As for RNA, it is becoming clear how important is its intracellular localization for the control of proper cell differentiation and development and how its perturbation can be linked to several pathological states. This aspect is even more important if one thinks of highly polarized cells su
发表于 2025-3-22 09:12:29 | 显示全部楼层
Sanger Sequencing to Determine the Full-Length Sequence of Circular RNAsies using high throughput RNA-sequencing (RNA-seq) techniques and novel computational programs reported the abundant and ubiquitous expression of circRNAs originating by pre-mRNA backsplicing. CircRNAs are mostly involved in gene expression by regulating functions of interacting microRNAs (miRNAs) a
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Targeted Sequencing of Circular RNAs for Illumina-Based Counting and Nanopore Structure Determinatioe same linear transcripts that are canonically spliced to produce, for example, mature mRNAs. They exhibit tissue-specific expression pattern and are potentially involved in many diseases, among them cardiovascular diseases. However, despite the tremendous efforts to establish circRNA catalogues, mu
发表于 2025-3-22 22:40:53 | 显示全部楼层
Nanopore-Mediated Sequencing of Circular RNAcircRNAs are composed of more than one exon, which are spliced together in a linear fashion. This protocol describes methods to sequence full-length circRNA across the back-splicing junction, allowing unambiguous characterization of circRNA-specific exon-intron structures by long-read sequencing (LR
发表于 2025-3-23 04:40:22 | 显示全部楼层
In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdow
发表于 2025-3-23 05:42:21 | 显示全部楼层
The Functional Circular RNA Screening via RfxCas13d/BSJ-gRNA Systemly. As circRNAs share overlapping sequences with their cognate linear RNAs, except for the back-splicing junction sites, it is difficult to distinguish circRNAs from cognate mRNAs in functional studies. In this chapter, we describe a programmable method for the large-scale functional circRNA screeni
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