Titlebook: Cilia; Methods and Protocol Peter Satir,Søren Tvorup Christensen Book 2016 Springer Science+Business Media New York 2016 intraflagellar tra

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Recombinant Reconstitution and Purification of the IFT-B Core Complex from ,,ocol for the purification and reconstitution of a complex containing nine . IFT proteins, commonly known as the IFT-B core complex. In our hands, this protocol routinely yields several milligrams of pure complex suitable for structural analysis by X-ray crystallography and single-particle cryo-electron microscopy.

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1064-3745 ation advice from the experts.This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging and

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Reformpotenzial von Regierungssystemen,bed that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology in healthy and pathophysiological conditions. Here, we describe methods to monitor autophagy activity in cilia mutants, as well as the use of autophagy mutants to monitor ciliogenesis.