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Titlebook: Cilia; Methods and Protocol Peter Satir,Søren Tvorup Christensen Book 2016 Springer Science+Business Media New York 2016 intraflagellar tra

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CLEM Methods for Studying Primary Cilia,zes image manipulation in correlating the fluorescent and SEM images. Alternative methods prepare samples for fluorescence imaging followed by processing for SEM then observation in the SEM. This method is ideal for optimal fluorescence imaging, particularly live cell imaging.
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Postkommunistische Regierungssystemvarianz,photoreceptor cells of native vibratome sections through the retina by high laser intensity. In the laser scanning microscope, the recovery of the fluorescent signal is monitored over time and the kinetics of movements of molecules can be quantitatively ascertained.
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Methods for Studying Ciliary Import Mechanisms,s into ciliated cells. We also describe a fluorescence recovery after photobleaching (FRAP) assay to measure the entry of ciliary proteins into the ciliary compartment. These assays can be used to determine the molecular mechanisms that regulate the formation and function of cilia in mammalian cells.
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