Titlebook: Cilia; Methods and Protocol Peter Satir,Søren Tvorup Christensen Book 2016 Springer Science+Business Media New York 2016 intraflagellar tra

退出可食用

Postkommunistische Regierungssystemvarianz,of motor molecules..Here, we describe in detail our method for measuring the motility of proteins in cilia or dendrites of . male-specific CEM ciliated sensory neurons using time-lapse microscopy and kymography of green fluorescent protein (GFP)-tagged motors, receptors, and cargos. We describe, as

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https://doi.org/10.1007/978-981-99-3272-6bined, these feature make planaria a useful model system in which to examine ciliary assembly and motility, especially in the context of a ciliated epithelium where many organelles beat in a hydrodynamically coupled synchronized manner. In addition, as planaria secrete mucus against which the cilia

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muscle-fibers

Targeting of ASH Domain-Containing Proteins to the Centrosome,ative immunofluorescence microscopy (IFM) analysis in cultured human telomerase-immortalized retinal pigmented epithelial (hTERT-RPE1) cells, we could confirm that TRAPPC13 and NPHP4 are highly enriched at the base of primary cilia, whereas DLEC1 seems to associate specifically with motile cilia. Re

infantile

Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in ,,of motor molecules..Here, we describe in detail our method for measuring the motility of proteins in cilia or dendrites of . male-specific CEM ciliated sensory neurons using time-lapse microscopy and kymography of green fluorescent protein (GFP)-tagged motors, receptors, and cargos. We describe, as

沙草纸

Methods for Studying Ciliary-Mediated Chemoresponse in ,,(1) assaying populations of cells for their behavioral responses to chemicals (attraction and repulsion), (2) characterization of the chemoreceptors and associated channels of the cilia using proteomics and binding assays, and (3) electrophysiological analysis of individual cells’ responses to chemi

HARP

, as a Model System for the Analysis of Ciliary Assembly and Motility,bined, these feature make planaria a useful model system in which to examine ciliary assembly and motility, especially in the context of a ciliated epithelium where many organelles beat in a hydrodynamically coupled synchronized manner. In addition, as planaria secrete mucus against which the cilia

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