Titlebook: Chromosome Analysis Protocols; John R. Gosden Book 1994 Humana Press 1994

fidelity

态度暖昧

Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity, also highlighted structural and functional differences between different chromosomal segments (.). Restriction endonucleases (RE) have been widely used to study some aspects of chromosome organization (.–.). The original methods, involving digestion followed by Giemsa or ethidium bromide (EB) stain

AMBI

Aboveboard

Bivariate Chromosome Analysis Using a Commercial Flow Cytometer,specificity for GC-rich DNA) was first described by Gray et al. in 1979 (.). Chromosomes stained with these two dyes can be resolved on the flow cytometer not only by their DNA content, but also by base pair ratio (..). Although other dye combinations have been used (e.g., refs. .–.), Hoechst and Ch

mettlesome

ANTE

催眠

Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations,cellular distribution of known antigens using defined antibodies, to aid in the characterization of novel antigens recognized, for example, by autoimmune sera, or to screen monoclonal antibodies for their cognate antigens, the immunofluorescence method has the merit of combining high sensitivity wit

Cupping

Immunocytochemical Techniques Applied to Meiotic Chromosomes,e appearance of meiosis-specific structures. Chromosome alignment is accompanied by the formation of axial elements along each chromosome (.); in meiocytes of some species, fibers appear to pull the axial elements of homologous chromosomes together (.). Alignment is followed by a process called syna

commune

Immortalized Cell Lines,span of the current genome mapping effort. Human geneticists contemplate mapping a gene as soon as its presence can be identified either directly at the DNA level or through its product (RNA, protein, or even an abnormal disease state). Unlike the situation in the mouse, physical rather than genetic

Allodynia

Oligonucleotide Primed in Situ DNA Synthesis (PRINS),t time, the capabihties of the method have expanded enormously. In the early years, the sensitivity (minimum size of target capable of being detected) was restricted to highly repeated DNA sequences, such as satellite DNAs, and detection was exclusively by autoradiography of radioisotopic label (e.g