Titlebook: Chromosome Analysis; Methods and Protocol Eisuke Gotoh Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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Analysis for Sister Chromatid Exchange,ages of SCE. This chapter will provide the detailed method for the SCE staining and measurement for the traditional FPG staining, BrdU monoclonal antibody staining method, and newly developed EdU Click reaction staining method.

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https://doi.org/10.1007/978-3-030-27957-8chromosome rearrangements that frequently occurred in cancer. Therefore, Hi-C is a powerful technique to analyze epigenomic and genomic aberrations in tumorigenesis. Here we will introduce the basic protocol of Hi-C in experimental and analytical aspects.

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Rosemarye T. Taylor,Nicole Lea Marshtein-A Tn5 transposase fusion protein cuts the genome and inserts adapter sequences nearby the target protein. Here we describe the updated CUT&Tag procedure using “home-made” con A-conjugated magnetic beads. This method is free of poor suspendability and severe aggregation, hence providing improved sensitivity.

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Chromosome Analysis978-1-0716-2433-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-1-0716-2433-3FISH; mitotic chromosome; Sister chromatid exchanges; Micronucleus; cytospin

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