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Titlebook: Chromosome Analysis; Methods and Protocol Eisuke Gotoh Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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书目名称Chromosome Analysis
副标题Methods and Protocol
编辑Eisuke Gotoh
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromosome Analysis; Methods and Protocol Eisuke Gotoh Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license t
描述.This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent .in situ. hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.. ..Authoritative and cutting-edge, .Chromosome Analysis: Methods and Protocols .aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
出版日期Book 2023
关键词FISH; mitotic chromosome; Sister chromatid exchanges; Micronucleus; cytospin
版次1
doihttps://doi.org/10.1007/978-1-0716-2433-3
isbn_softcover978-1-0716-2435-7
isbn_ebook978-1-0716-2433-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Preparation of Mitotic Cells for Fluorescence Microscopy,ensation, alignment, and segregation in M phase. These beautiful behaviors of chromosomes observed through the microscope have fascinated people since more than 100 years ago, and now we can sketch the dynamics of regulatory proteins and their posttranscriptional modifications through the fluorescen
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Chemical-Induced Premature Chromosome Condensation Protocol, by colcemid block protocol. Premature chromosome condensation (PCC) technique is an alternative to obtain chromosomes. It was more than half century ago that the first observation of PCC phenomena reported. Since then, cell-fusion-mediated PCC method has been developed and introduced in many fields
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Apoptosis Detection Assays,apoptosis accurately, two or more assays should be used since there are many overlapped features between apoptosis and necrosis. There are six major groups of available assays to detect apoptosis: membrane alteration, mitochondrial assays, cytomorphological alterations, DNA fragmentation, detection
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Alkaline Comet Assay to Detect DNA Damage,resis, this assay is especially simplistic, and the results are easily reproducible. DNA breakage can be quantitatively assessed by the induced comet tail regions, which can be measured using a variety of comet software. This protocol will finish within approximately two hours with adequate preparat
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Analysis for Sister Chromatid Exchange,ered as a result of replication stress from genetic defects, ROS stress, and genomic damages. SCE staining needs extra processes compared to regular Giemsa staining. Usually two rounds of cell cycle progress are required to observe SCE under microscope. SCE can be visualized with the fluorescence pl
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Cytokinesis Blocked Micronuclei Aberration Analysis,notoxic agent exposure produces DNA damage and chromosome fragments. Fragmented chromosomes without centromere failed to attach kinetochore which segregates a pair of homologous chromosomes to each daughter cells at cytokinesis, hence leading to form micronuclei. Chromosome or fragments of chromosom
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