Titlebook: Chromosome Analysis; Methods and Protocol Eisuke Gotoh Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license t

畸形

FISH with Whole Chromosome Painting Probes, person’s eye. Whole chromosome painting FISH probe can stain designated entire chromosomes or domains in metaphase chromosomes or interphase nuclei, respectively. It allows to visually identify translocations, deletions, or amplifications of specific chromosomes. Once chromosomes are stained, even

连系

outrage

Nontraditional Method for Telomere Staining by PNA Probes,time to obtain detectable signals compared to other reactions such as immunochemical reactions and simple chemical reactions. Certain proteins bind to specific DNA sequences and regulate the biological function of DNA. These DNA-binding proteins have specific domains to interact with single- or doub

Promotion

Visualizing Active Replication Regions in S-Phase Chromosomes,proceeds tightly coupled with DNA replication progresses. Drug-induced PCC is a useful tool for visualization of the interphase nuclei, in particular, S-phase, as S-phase prematurely condensed chromosomes (S-phase PCC). Active replicating DNA is labeled directly with Cy3-dUTP by bead loading method,

Brain-Imaging

Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer,ption or replication, and Hi-C has revealed the hierarchical chromatin structures, such as loop, domain, and compartment structures. Aberrant alteration of these structures causes disease, and a number of structural aberrations in cancer cells have been reported recently. Besides, Hi-C can identify

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折磨

Histone Modification Analysis of Low-Mappability Regions,difications including acetylation, methylation, ubiquitination, and SUMOylation of lysine or arginine residue of nucleosome core histones in gene expression have been intensively studied. Genome-wide profiles of histone modification marks revealed their combinatorial organization in the functional f

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用树皮

FISH with Whole Chromosome Painting Probes,non-skilled researchers can easily identify those chromosome aberrations. Whole chromosome painting FISH has higher sensitivity to detect chromosome aberrations. This chapter introduces the method for whole chromosome painting FISH staining.