Titlebook: Chromosomal Mutagenesis; Shondra M. Pruett-Miller Book 2015Latest edition Springer Science+Business Media New York 2015 Genome editing too

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Redefining Chinese Literature and Art virtually any location in the human genome within 50 bp of a desired site. Such high resolution ZFN engineering is well within the conversion tract limitations demarcated by the mammalian DNA repair machinery, resulting in a nearly universal ability to create point mutations throughout the human ge

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Redefining Chinese Literature and Artered regularly interspaced short palindromic repeats (CRISPR)/Cas9 nucleases, requires the creation of a targeted, chromosomal DNA double-stranded break (DSB). In mammalian cells, these DSBs are typically repaired by one of the two major DNA repair pathways: nonhomologous end joining (NHEJ) or homol

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Redefining Chinese Literature and Arten reported. Nonetheless, an important practical aspect of the strategy is develop methods to increase the frequency of genome editing or methods that enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of

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Adele Eskeles Gottfried,Allen W. Gottfriedargeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create

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https://doi.org/10.1007/978-1-4899-0961-9 engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without

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Adele Eskeles Gottfried,Allen W. Gottfriedigerator at 4 °C. Furthermore, it is possible to realize easy and safe transportation of spermatozoa at an ambient temperature that requires neither liquid nitrogen nor dry ice. Freeze-drying spermatozoa has been established as a new method for storing genetic resources instead of cryopreservation u

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Shondra M. Pruett-MillerIncludes all new cutting-edge methods and protocols for chromosomal mutagenesis research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr