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Titlebook: Chromosomal Mutagenesis; Shondra M. Pruett-Miller Book 2015Latest edition Springer Science+Business Media New York 2015 Genome editing too

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发表于 2025-3-21 16:31:33 | 显示全部楼层 |阅读模式
书目名称Chromosomal Mutagenesis
编辑Shondra M. Pruett-Miller
视频video
概述Includes all new cutting-edge methods and protocols for chromosomal mutagenesis research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromosomal Mutagenesis;  Shondra M. Pruett-Miller Book 2015Latest edition Springer Science+Business Media New York 2015 Genome editing too
描述.This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and fully updated, .Chromosomal Mutagenesis, Second Edition. aims to help speed scientific discovery and aid in the next advances in the field.
出版日期Book 2015Latest edition
关键词Genome editing tools; Genome engineering; Homologous recombination; Mammalian systems; Model organisms; M
版次2
doihttps://doi.org/10.1007/978-1-4939-1862-1
isbn_softcover978-1-4939-5541-1
isbn_ebook978-1-4939-1862-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy,C31 integrase performs efficient recombination between its . site and either its own placed . site or a partially mismatched genomic pseudo . site. Bxb1 integrase, another large serine recombinase, has a similar level of recombinational activity, but recognizes only its own . and . sites. Previously
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Genome Engineering Using Adeno-Associated Virus (AAV),sease biology. These techniques fall into two categories based on the DNA repair mechanism that is used to incorporate the genetic change. Nuclease-based technologies, such as Zinc-Finger Nucleases, TALENS, and Crispr/Cas9, rely on non-homologous end-joining (NHEJ) and homology directed repair (HDR)
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Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform, domain and the development of TALE .ucleases (TALENs). TALENs enable researchers to make DNA double-strand breaks in target loci to create gene knockouts or introduce specific DNA sequence modifications. Precise genome engineering is increasingly being used to study gene function, develop disease m
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Ligation-Independent Cloning (LIC) Assembly of TALEN Genes,s, e.g. using the . nuclease domain, provides a potent tool to induce DNA double strand breaks at user-defined genomic loci. In this regard, TAL (transcription activator-like) effector proteins, secreted by bacteria of the Xanthomonas family, provide the highest degree of modularity in their DNA bin
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Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications,TALs are novel monomeric nucleases composed of a site-specific meganuclease cleavage head with additional affinity and specificity provided by a TAL effector DNA binding domain. This fusion product facilitates the transformation of meganucleases into hyperspecific and highly active genome engineerin
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