Titlebook: Chromosomal Mutagenesis; Shondra M. Pruett-Miller Book 2015Latest edition Springer Science+Business Media New York 2015 Genome editing too

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Book 2015Latest editiontheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and fully updated, .Chromosomal Mutagenesis, Second Edition. aims to help speed scientific discovery and aid in the next advances in the field.

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International and Development EducationEN construction based on one of the most popular TALEN assembly platforms, the Golden Gate cloning method. Using this protocol, ready-to-use TALENs with specificity for targets 13–32 bp long are constructed within 5 days.

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Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy,integrases simultaneously to facilitate cassette exchange at a predefined location. This approach permits greater control and accuracy over integration. We also present a general method for using polymerase chain reaction assays to verify that the desired cassette exchange occurred successfully.

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Genome Engineering Using Adeno-Associated Virus (AAV),rations from gene knockouts, insertions of point mutations, indels, epitope tags, and reporter genes. Here we describe the generation and use of AAV gene targeting vectors and viruses to create targeted isogenic cell lines.

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Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform,EN construction based on one of the most popular TALEN assembly platforms, the Golden Gate cloning method. Using this protocol, ready-to-use TALENs with specificity for targets 13–32 bp long are constructed within 5 days.

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