Titlebook: Chimeric RNA; Methods and Protocol Hui Li,Justin Elfman Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Gene f

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NanoString nCounter Technology: High-Throughput RNA Validationation, single nucleotide variation and chimeric RNAs can be analyzed. In this chapter, we discuss the design, setup, and performance of a NanoString-based RNA assay for gene expression or fusion transcript assessment.

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Transport of Radioactive Materials,e driving the development of the INTEGRATE tool and describe the detailed practical procedures for applying INTEGRATE to discover gene fusions using NGS data. INTEGRATE can be applied to both combined data and RNA-seq only data.

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Ravinder Nath PhD,M. Saiful Huq PhDof chimeric RNAs in these fractions. Within this chapter we describe the most frequently used cellular fractionation technique: the separation of the cytoplasmic fraction from the nuclear fraction in a cell.

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RNase Protection Assay can bind specifically with the target chimeric RNA in order to form double-stranded RNA. This newly formed RNA is resistant to digestion by RNase and therefore can be identified by high-resolution, denaturing polyacrylamide gel electrophoresis.

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Overexpression of Chimeric RNA by Retroviral Transductionion of the chimeric RNA. Here, we provide procedures for the insertion of the . fusion ORF into a mammalian expression vector, achieving the overexpression of the . fusion protein in a colon cancer cell line by retroviral transduction.