Titlebook: Chimeric RNA; Methods and Protocol Hui Li,Justin Elfman Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Gene f

虚情假意

https://doi.org/10.1007/978-1-4939-9904-0Gene fusion; Transcription-mediated fusions; Fusion transcripts; Bioinformatics; Translational applicati

哄骗

978-1-4939-9906-4Springer Science+Business Media, LLC, part of Springer Nature 2020

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EFFECTS OF CONTAMINANT EXPOSURE ON PLANTSpackages predict a large number of false positive events, which significantly hampers experimental validation of predicted chimeric RNAs. Here, we describe the detailed steps for (1) prediction of chimeric RNAs using EricScript software, (2) characterization of chimeric RNAs to discard most probable

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ROOF

Transport of Radioactive Materials,ity of patients with whole transcriptome sequencing (RNA-seq) and whole genome sequencing (WGS) data. We developed a gene fusion discovery tool, INTEGRATE, that leverages both RNA-seq and WGS data to reconstruct gene fusion junctions and genomic breakpoints by split-read alignment. INTEGRATE has bec

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Transportation of Radioactive Material,scripts. RT-PCR consists of RNA isolation and reverse transcription into complementary DNA (cDNA), which can then be used as input to a variety of PCR-based procedures such as standard PCR, real-time or quantitative PCR (qPCR or qRT-PCR), or TaqMan PCR procedures. These assays are useful in detectio

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Alcove

Biological Effects of Radiation in Humans,s principle, the RNase Protection Assay (RPA) is used to validate chimeric RNAs. Importantly, this assay does not employ reverse transcription (RT), thus avoiding potential false-positive results which could occur during RT such as template-switching. We first generate RNA probes with .phosphate (P)