Titlebook: Cellular Senescence; Methods and Protocol Marco Demaria Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 senesc

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DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA),sensitive detection of physical DSBs in fixed cells, through direct labeling of the DSBs with biotinylated oligonucleotides, and subsequent signal amplification by PLA between biotin and a partner protein in the proximity of the DNA break.

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Mouse Models of Accelerated Cellular Senescence,n vivo remains challenging, as there is no marker unique to senescent cells. Here, we describe multiple methods to detect the presence and extent of cellular senescence in preclinical models, with a special emphasis on murine models of accelerated aging that exhibit a more rapid onset of cellular senescence.

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Book 2019nts, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .Cellular Senescence: Methods and Protocols .aims to ensure successful results in the further study of this vital field..

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A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescpreviously described assay, developed for in vitro and in vivo senescent cell recognition that exploits a newly synthesized Sudan Black-B analog (GL13). Analysis of human clinical samples with the modified protocol provided strong evidence of its usefulness for the exposure and surveillance of age-related conditions.

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Reactive Oxygen Species Detection in Senescent Cells,ment and ageing. Senescent cells are characterized by increased production of reactive oxygen species (ROS), mostly produced by dysfunctional mitochondria. Both intracellular and extracellular ROS have been shown to contribute to the induction of senescence. ROS have also been shown to act as signal

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,Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Viv cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol