Titlebook: Cellular Senescence; Methods and Protocol Marco Demaria Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 senesc

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Superresolution in Scanning Optical Systemsescence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of . expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.

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Measuring the Inflammasome in Oncogene-Induced Senescence,escence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of . expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.

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Neural Control of Ocular Blood Flowing molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.

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Tim M. Curtis Ph.D.,Tom A. Gardiner Ph.D. 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.

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Superresolution in Scanning Optical Systems results in a coordinated induction of senescence, recapitulating different aspects of the phenotype such as the growth arrest and the establishment of a senescence-associated secretory phenotype (SASP).

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Book 2019 heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells.  Written in the highly successf

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https://doi.org/10.1007/978-3-540-69565-3 our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3–4 days starting from single cell isolation to analysis of sequencing data.