Titlebook: Cellular Senescence; Methods and Protocol Marco Demaria Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 senesc

LARK

Endothelial and Adrenergic ControlCellular senescence has been suggested to facilitate tissue regeneration via promoting cellular plasticity. Here, we describe multiple systems, both in vitro and in vivo, to detect senescence in the context of cellular reprogramming.

单独

Acetabulum

Marco DemariaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

paleolithic

施魔法

Cellular Senescence978-1-4939-8931-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

责问

Superresolution in Scanning Optical Systemse. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.

浮雕

Reactive Oxygen Species Detection in Senescent Cells,ing molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.

惊奇

,Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Viv 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.

helper-T-cells

IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence, results in a coordinated induction of senescence, recapitulating different aspects of the phenotype such as the growth arrest and the establishment of a senescence-associated secretory phenotype (SASP).

津贴

1064-3745 ation advice from the experts.This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Addition