Titlebook: Cellular Senescence; Methods and Protocol Marco Demaria Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 senesc

Pcos971

Relative Human Telomere Length Quantification by Real-Time PCR, The method has been developed by Cawthon in 2002 (Cawthon, Nucleic Acids Res 30:47e–47, 2002) and remains the most frequently used technique either in original or modified version. Telomere length is estimated by comparing the amount of telomere repeat amplification product (T) to a single copy gen

Gnrh670

Assessing Functional Roles of the Senescence-Associated Secretory Phenotype (SASP),ted with age-related pathologies such as cancer progression. Numerous functions of senescent cells depend on their ability to secrete bioactive molecules, a characteristic termed the senescence-associated secretory phenotype (SASP). Although the SASP is generally described as proinflammatory, its tr

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TRAWL

IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence, suppressor mechanism limiting cancer progression. Here we describe IMR90 ER:RAS, a widely used model to study OIS in cell culture. This model takes advantage of IMR90 human primary fibroblast infected with a 4-hydroxy-tamoxifen (4-OHT) inducible ER:RAS construct. RAS activation upon 4-OHT treatment

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Genotoxic Stress-Induced Senescence,hemotherapy, apply. If the DNA repair machinery fails to fix the damaged site during a temporary cell-cycle arrest, or if massive genotoxic stress overwhelmed the repair capacity, cellular failsafe programs such as apoptosis or senescence will be triggered to limit aberrant propagation of these dama

CUB

A Multiparametric Assay to Evaluate Senescent Cells,with age and are present at sites of tissue damage and age related pathologies. However, the characterization of senescence cells in vivo is currently limited and the need for new technologies to detect and monitor the senescence state in vivo has greatly increased. Here we demonstrate the use of th

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Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry,e. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.

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