Titlebook: Catharanthus roseus; Methods and Protocol Vincent Courdavault,Sebastien Besseau Book 2022 The Editor(s) (if applicable) and The Author(s),

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https://doi.org/10.1007/978-3-319-43249-6ering enzymes involved in MIA biosynthesis. Here, we discuss the latest progress in understanding the roles of oxidative enzymes in MIA metabolism and describe a bioinformatic and biochemical pipeline to identify, characterize, and make use of these plant biocatalysts.

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William J. McGuire,Carina J. Fearnleyl expression as the pathway genes, and (3) isolation of homologs of TFs known to regulate structurally similar or diverse specialized metabolites in different plant species. TFs regulating TIA pathway have been isolated using either an individual or a combination of the three approaches. Here we des

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Book 2022 reproducible protocols.. Authoritative and cutting-edge, .Catharanthus roseus: Methods and Protocols .aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms.

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https://doi.org/10.1007/978-3-319-44144-3omoter transactivation studies or transgene overexpression paired with downstream analyses like quantitative PCR or metabolite analysis. Our protocol takes about 16 days from sowing seeds to obtaining the results of the experiment.

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https://doi.org/10.1007/978-3-319-44144-3st, easy to perform, and results in high amount of transient expression in .. This protocol is expected to serve as valuable tool to enhance the in planta characterization of gene functions or even transiently knock-in novel enzymatic activities.

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A Rapid and Efficient Vacuum-Based Agroinfiltration Protocol for Transient Gene Overexpression in Lst, easy to perform, and results in high amount of transient expression in .. This protocol is expected to serve as valuable tool to enhance the in planta characterization of gene functions or even transiently knock-in novel enzymatic activities.

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1064-3745 ation advice from the experts.This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in .C. roseus. from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor chara