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Titlebook: Catharanthus roseus; Methods and Protocol Vincent Courdavault,Sebastien Besseau Book 2022 The Editor(s) (if applicable) and The Author(s),

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,Semisynthesis of Bis-Indole Alkaloid (−)-Melodinine K Enabled by a Combination of Biotransformationmodified . yeast strain expressing a tabersonine 16-hydroxylase enzyme to enable regioselective oxidation on multigram scale, and (−)-pachysiphine is produced through stereoselective and regioselective epoxidation of the disubstituted alkene.
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Discovery and Characterization of Oxidative Enzymes Involved in Monoterpenoid Indole Alkaloid Biosyering enzymes involved in MIA biosynthesis. Here, we discuss the latest progress in understanding the roles of oxidative enzymes in MIA metabolism and describe a bioinformatic and biochemical pipeline to identify, characterize, and make use of these plant biocatalysts.
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Marloes Lamain,Jaap Scheerens,Peter Noortidentified biosynthetic steps can be used as baits to capture the remaining unknown steps. The present protocol shows how supervised machine learning in the form of artificial neural networks (ANNs) can efficiently classify genes as specialized metabolism related or not according to their expression
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