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Titlebook: Catharanthus roseus; Methods and Protocol Vincent Courdavault,Sebastien Besseau Book 2022 The Editor(s) (if applicable) and The Author(s),

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Chris McKee,Ima Itikarai,Hugh Daviesase resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal p
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https://doi.org/10.1007/978-3-319-44144-3lant . (Apocynaceae). No stable transformation and regeneration of plantlets have been achieved with a high efficiency in this plant to date. In addition, while virus-mediated transient gene silencing has been reported a decade ago in ., tools for transient overexpression remain scarce. Here, we des
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https://doi.org/10.1007/978-3-319-43908-2Transcriptional regulation is a central piece of the highly valuable monoterpenoid indole alkaloid pathway of .. and the ultimate tool for its understanding and manipulation. Here, we describe the adaptation of the TARGET methodology to identify specific and genome-wide leaf targets of . candidate transcription factors (TFs).
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Chris McKee,Ima Itikarai,Hugh Daviesase resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant ..
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RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation ole of most of the nearly 30 genes involved in this pathway. We introduce here a comparison of advantages and drawbacks of in situ hybridization and more popular promoter: GUS strategies. Two main advantages of in situ hybridization are the suitability to any plant species and the direct localization
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